nebuilder hifi dna assembly master mix (New England Biolabs)
New England Biolabs is a verified supplier 
New England Biolabs manufactures this product 
99
Structured Review
New England Biolabs
nebuilder hifi dna assembly master mix
Nebuilder Hifi Dna Assembly Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 9253 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nebuilder+hifi+assembly/NEBuilder+HiFi+DNA+Assembly+Master+Mix/pmc13049409-215-0-6
Average 99 stars, based on 9253 article reviews
Nebuilder Hifi Dna Assembly Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 9253 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nebuilder+hifi+assembly/NEBuilder+HiFi+DNA+Assembly+Master+Mix/pmc13049409-215-0-6
Average 99 stars, based on 9253 article reviews
nebuilder hifi dna assembly master mix - by Bioz Stars,
2026-09
99/100 stars
Images
Related Articles
Construct:Article Title: Phase separation of mycobacterial Rho factor is associated with acid stress Article Snippet: .. Plasmids were constructed with Polymerase Chain Reaction:Article Title: Increased Telomere Mobility in Progeria is Restored by Isoprenylcysteine Carboxyl Methyltransferase Inhibition Article Snippet: First, amplicons of mStayGold and TRF2 containing compatible overhangs for Gibson assembly were generated by PCR (NEB, M0492S) from plasmids pcDNA3/F-tractin=mStayGold (Addgene #212019, a gift from Atsushi Miyawaki) and pCIBN-tagBFP-hTRF2 (Addgene #103803, a gift from Karsten Rippe), respectively. .. The two fragments were assembled into a PCR-linearized third-generation lentiviral transfer vector backbone after the Ubc promoter using Article Title: Robust fluorescent labeling and tracking of endogenous non-repetitive genomic loci Article Snippet: Miniprepped plasmids were checked for size by restriction digest with BamHI-HF (NEB, R3136S) and XhoI (NEB, R0146S) and then confirmed by full-plasmid sequencing (Plasmidsaurus). .. To generate lentiviral transfer vectors, the DAP array was digested with BamHI-HF and XhoI, the correct band excised from a gel, and inserted into a PCR-amplified 3 rd generation lentiviral transfer vector containing a puromycin resistance cassette (pAR220 in Supplementary Table 6, which was derived from pLH-spsgRNA2 (Addgene #64114, gift from Dr. Thoru Pederson)), containing overhangs matching the ends of the DAP array using Article Title: Robust fluorescent labeling and tracking of endogenous non-repetitive genomic loci Article Snippet: All primers were ordered from Integrated DNA Technologies, Inc. (IDT) as ssDNA oligonucleotides. .. For DAP array cloning: the vector and fragment template plasmids were obtained (hCtRNA_VT (Addgene #186716) and hCtRNA_FT (Addgene #186715), gifts from Dr. Xue Gao) and the sgRNA scaffold was replaced with the optimized scaffold for dSaCas9 by cloning the dSaCas9-optimized scaffold, which was ordered as dsDNA in a gBlock (IDT), into the PCR-amplified template (either hCtRNA_VT or hCtRNA_FT) using Plasmid Preparation:Article Title: Increased Telomere Mobility in Progeria is Restored by Isoprenylcysteine Carboxyl Methyltransferase Inhibition Article Snippet: First, amplicons of mStayGold and TRF2 containing compatible overhangs for Gibson assembly were generated by PCR (NEB, M0492S) from plasmids pcDNA3/F-tractin=mStayGold (Addgene #212019, a gift from Atsushi Miyawaki) and pCIBN-tagBFP-hTRF2 (Addgene #103803, a gift from Karsten Rippe), respectively. .. The two fragments were assembled into a PCR-linearized third-generation lentiviral transfer vector backbone after the Ubc promoter using Article Title: Spectral Tuning in Mammalian Melanopsins Article Snippet: Gene sequences were synthesized using ThermoFisher GeneArt Gene Fragment synthesis and TwistBio Gene Fragment synthesis, with codon optimization where necessary for synthesis. .. Gene sequences were inserted into the multiple cloning site of the pcDNA3 plasmid vector (Invitrogen, Cat# V79020) using Article Title: Spectral Tuning in Mammalian Melanopsins. Article Snippet: Gene sequences were synthesized using ThermoFisher GeneArt Gene Fragment synthesis and TwistBio Gene Fragment synthesis, with codon optimization where necessary for synthesis. .. Gene sequences were inserted into the multiple cloning site of the pcDNA3 plasmid vector (Invitrogen, Cat# V79020) using Article Title: Disturbed mitochondrial maturation in cardiolipin remodeling-deficient cardiomyocytes Article Snippet: .. WT TAZ or H69Q mutant, as we previously reported, were subcloned into the pFUGW backbone plasmid (EF-1α promoter) by Transformation Assay:Article Title: Increased Telomere Mobility in Progeria is Restored by Isoprenylcysteine Carboxyl Methyltransferase Inhibition Article Snippet: First, amplicons of mStayGold and TRF2 containing compatible overhangs for Gibson assembly were generated by PCR (NEB, M0492S) from plasmids pcDNA3/F-tractin=mStayGold (Addgene #212019, a gift from Atsushi Miyawaki) and pCIBN-tagBFP-hTRF2 (Addgene #103803, a gift from Karsten Rippe), respectively. .. The two fragments were assembled into a PCR-linearized third-generation lentiviral transfer vector backbone after the Ubc promoter using Derivative Assay:Article Title: Robust fluorescent labeling and tracking of endogenous non-repetitive genomic loci Article Snippet: Miniprepped plasmids were checked for size by restriction digest with BamHI-HF (NEB, R3136S) and XhoI (NEB, R0146S) and then confirmed by full-plasmid sequencing (Plasmidsaurus). .. To generate lentiviral transfer vectors, the DAP array was digested with BamHI-HF and XhoI, the correct band excised from a gel, and inserted into a PCR-amplified 3 rd generation lentiviral transfer vector containing a puromycin resistance cassette (pAR220 in Supplementary Table 6, which was derived from pLH-spsgRNA2 (Addgene #64114, gift from Dr. Thoru Pederson)), containing overhangs matching the ends of the DAP array using Cloning:Article Title: Spectral Tuning in Mammalian Melanopsins Article Snippet: Gene sequences were synthesized using ThermoFisher GeneArt Gene Fragment synthesis and TwistBio Gene Fragment synthesis, with codon optimization where necessary for synthesis. .. Gene sequences were inserted into the multiple cloning site of the pcDNA3 plasmid vector (Invitrogen, Cat# V79020) using Article Title: Spectral Tuning in Mammalian Melanopsins. Article Snippet: Gene sequences were synthesized using ThermoFisher GeneArt Gene Fragment synthesis and TwistBio Gene Fragment synthesis, with codon optimization where necessary for synthesis. .. Gene sequences were inserted into the multiple cloning site of the pcDNA3 plasmid vector (Invitrogen, Cat# V79020) using Article Title: Robust fluorescent labeling and tracking of endogenous non-repetitive genomic loci Article Snippet: All primers were ordered from Integrated DNA Technologies, Inc. (IDT) as ssDNA oligonucleotides. .. For DAP array cloning: the vector and fragment template plasmids were obtained (hCtRNA_VT (Addgene #186716) and hCtRNA_FT (Addgene #186715), gifts from Dr. Xue Gao) and the sgRNA scaffold was replaced with the optimized scaffold for dSaCas9 by cloning the dSaCas9-optimized scaffold, which was ordered as dsDNA in a gBlock (IDT), into the PCR-amplified template (either hCtRNA_VT or hCtRNA_FT) using Mutagenesis:Article Title: Disturbed mitochondrial maturation in cardiolipin remodeling-deficient cardiomyocytes Article Snippet: .. WT TAZ or H69Q mutant, as we previously reported, were subcloned into the pFUGW backbone plasmid (EF-1α promoter) by Expressing:Article Title: A thyroid hormone–mediated opsin switch initiates metamorphosis in a proto-vertebrate Article Snippet: Subsequently, the T2A-mNG sequence was subcloned downstream of Cas9 using NEBuilder HiFi assembly, generating the Nkx2.1>nls::Cas9::nls::T2A:mNG construct. .. For photoreceptor-specific expression of Cas9, the Opsin1 enhancer was cloned upstream of the Cas9 coding region using Clone Assay:Article Title: A thyroid hormone–mediated opsin switch initiates metamorphosis in a proto-vertebrate Article Snippet: Subsequently, the T2A-mNG sequence was subcloned downstream of Cas9 using NEBuilder HiFi assembly, generating the Nkx2.1>nls::Cas9::nls::T2A:mNG construct. .. For photoreceptor-specific expression of Cas9, the Opsin1 enhancer was cloned upstream of the Cas9 coding region using |